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High Chemical Resolution Imaging using Gas-phase Ion/Ion Reactions
Monday, March 08, 2021: 2:40 PM - 3:15 PM
Speaker(s)
Description
Imaging mass spectrometry (IMS) is a powerful technology that enables the visualization of biochemical processes directly in tissues by combining the molecular specificity of mass spectrometry with the spatial fidelity of microscopic imaging. Especially when studying lipids, there are many isobaric and isomeric molecules that complicate spectral analysis, with each isoform having a potentially unique cellular function. While traditional tandem mass spectrometry (MS/MS) approaches can distinguish amongst these compounds in some instances, this is often not the case. Additionally, the ion type most readily generated from tissue is rarely the type that affords the most chemical structural information upon MS/MS. Our lab is developing gas-phase reactions that afford the ability to transform the ion type without manipulating the sample, making these reactions ideally suited for implementation into IMS workflows to enable novel structural identification and separations based on chemical reactivity. We have enabled gas-phase charge inversion ion/ion reactions on a hybrid QhFT-ICR mass spectrometer. Phosphatidylcholine cations generated from tissue using matrix-assisted laser desorption/ionization (MALDI) and doubly deprotonated 1,4-phenylenedipropionic acid reagent anions generated via electrospray ionization (ESI) are sequentially injected into a hexapole reaction cell. Following a mutual storage reaction period (~750 ms), the product ions are are subjected to MS/MS and then mass analyzed. This technology has enabled the identification of multiple sn-positional isomers directly from mouse brain tissue, the relative contributions of which vary significantly between regions of the brain. This technology has also been used to separate isobaric PC lipids as well as concentrate ion signal from +H, +Na, and +K ion types. Finally, our group is exploring other gas-phase chemistries to enable lipid double bond localization and lipid class differentiation.
Additional Info
Keywords: Please select up to 4 keywords ONLY:
Biomedical,Instrumentation,Laser Desorption (MALDI),Lipids
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